GeneJET Plasmid Miniprep Kit from Thermo Fisher Scientific

Product Specs
ItemGeneJET Plasmid Miniprep Kit
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberK0503
Quantity250 preps
ApplicationsPCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
FormatSpin Column
TargetPlasmid DNA
Brand/TrademarkThermo Scientific™
TypePlasmid MiniPrep Kit, Spin Column
Sample TypeBacteria
Time<14 min

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Protocol for single-molecule labeling and tracking of bacterial cell division proteinsSTAR ProtocDecember 12, 2023Di Yan, Jinchan Xue, Jie Xiao, Zhixin Lyu, Xinxing Yangassays ,, In-Fusion HD Cloning Kit,Takara,639650 GeneJET Plasmid Miniprep Kit,Thermo Fisher Scientific,K0503 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Fiji or ImageJ,Fiji

(2) Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, EgyptWorld Journal of Microbiology and BiotechnologyDecember 1, 2023Azza S. El-Demerdash, Rehab E. Mowafy, Hanan A. Fahmy, Ahmed A. Matter, Mohamed Samirbacterial isolates using Thermo Scientific GeneJET Plasmid Miniprep Kit (Thermo, Germany, Catalogue no. K0503) for detection of antimicrobial resistant genes. PCR amplification of virulence-related genes of P.

(3) Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regenerationeLifeNovember 21, 2023Laura Celotto, Fabian Rost, Anja Machate, Juliane Bläsche, Andreas Dahl, Anke Weber, Stefan Hans, Michael Brand(1:2000) overnight at 37 °C, shaking, to enrich cultures for mini-prep (Gene Jet Plasmid Miniprep kit k0503, Thermo Fisher). Purified plasmids were sent for sequencing (Eurofins) to verify the sequence. Insert-positive

(4) Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA Recombinase Sites for the Functional Characterization of Protein VariantsACS Synthetic BiologyNovember 17, 2023Sarah M. Roelle, Nisha D. Kamath, Kenneth A. Matreyekdilution plating and colony count. Plasmids were extracted with a GeneJet miniprep kit (Thermo Fisher, K0503) and sequence-confirmed via Sanger sequencing on an Applied Biosystems 3730 genetic analyzer, followed

(5) Atypical Protein Kinase C Promotes its own Asymmetric Localisation by Phosphorylating Cdc42 in Polarising CellsbioRxivNovember 2, 2023John Packer, Alicia G. Gubieda, Aaron Brooks, Lars N. Deutz, Iolo Squires, Shona Ellison, Sundar Ram Naganathan, Adam J.M. Wollman, Daniel J. Dickinson, Josana RodriguezpDONR221-gCDC42attb. The plasmid was introduced by heat shock into DH5alpha and extracted with standard Miniprep (#K0503, ThermoFisher). The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with

(6) An engineered interleukin‐11 decoy cytokine inhibits receptor signaling and proliferation in lung adenocarcinomaBioengineering & Translational MedicineNovember 1, 2023Brianna J. McIntosh, Griffin G. Hartmann, Sean A. Yamada‐Hunter, Phillip Liu, Camille F. Williams, Julien Sage, Jennifer R. CochranZymo Research Corp), transformed into DH10B electrocompetent cells, and isolated using a plasmid miniprep kit (K0503, Thermo Fisher). Sequencing was performed by MCLAB. 5.2.2 gp130/IL‐11R screening Yeast displaying

(7) The genetics of aerotolerant growth in an alphaproteobacterium with a naturally reduced genomemBioOctober 31, 2023Amy L. Enright, Amy B. Banta, Ryan D. Ward, Julio Rivera Vazquez, Magdalena M. Felczak, Michael B. Wolfe, Michaela A. TerAvest, Daniel Amador-Noguez, Jason M. PetersE. coli strain BW25141 (sJMP146). Plasmids were purified using the GeneJet Plasmid Miniprep Kit (Thermo K0503), the QIAprep Spin Miniprep Kit (Qiagen 27106), or the Purelink HiPure Plasmid Midiprep Kit (Invitrogen

(8) Glycosylation Circuit Enables Improved Catalytic Properties for Recombinant Alkaline PhosphataseACS OmegaOctober 3, 2023Eray Ulaş Bozkurt, İrem Niran Çağıl, Ebru Şahin Kehribar, Musa Efe Işılak, Urartu Özgür Şafak Şekerantibiotics. Plasmids were isolated using a Gene-JET Miniprep Kit (Thermo Scientific, Waltham USA, K0503). Isolated plasmids were verified by Sanger sequencing. Details of the amino-acid sequences of the cloned

(9) Impact of CRISPR/Cas9-Mediated CD73 Knockout in Pancreatic CancerCancers (Basel)October 3, 2023Jinping Zhang, Shuman Zhang, Isabella Dörflein, Xiaofan Ren, Susanne Pfeffer, Nathalie Britzen-Laurent, Robert Grützmann, Xianglong Duan, Christian Pilarskyclone, 10 bacterial colonies were chosen, and plasmid DNA was isolated (GeneJET Plasmid Miniprep Kit, K0503, Thermo Fisher, Langenselbold, Germany) for sequencing in Eurofins Genomics. 2.9. Quantitative PCR Total

(10) AP profiling resolves co-translational folding pathway and chaperone interactions in vivobioRxivSeptember 8, 2023Xiuqi Chen, Christian M. KaiserEZC106N) was used for DNA purification protocols. GeneJET plasmid miniprep kit (Thermo Scientific, K0503) was used for all plasmid miniprep steps. GeneJET PCR purification kit (Thermo Scientific, K0701) was

(11) Tools for Genetic Engineering and Gene Expression Control in Novosphingobium aromaticivorans and Rhodobacter sphaeroidesbioRxivAugust 31, 2023Ashley N. Hall, Benjamin W. Hall, Kyle J. Kinney, Gabby G. Olsen, Amy B. Banta, Daniel R. Noguera, Timothy J. Donohue, Jason M. Petersprepared from E. coli strains with one of the following kits: GeneJet plasmid miniprep kit (catalog number K0503; Thermo Scientific). QIAprep Spin Miniprep Kit (catalog number 27104; Qiagen), or the PureLink HiPure Plasmid Midiprep kit

(12) The Genetic Landscape of a Metabolic InteractionbioRxivAugust 23, 2023Thuy N. Nguyen, Christine Ingle, Samuel Thompson, Kimberly A. Reynolds10 ml of the overnight culture was miniprepped with the Gene-Jet Mini-prep kit (Fisher Scientific, K0503) to obtain the plasmid library. Growth Rate Measurements in the Turbidostat for all DHFR mutant libraries All

(13) Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxivJune 19, 2023Andrew J. Stout, Xiaoli Zhang, Sophia M. Letcher, Miriam L. Rittenberg, Michelle Shub, Kristin M. Chai, Maya Kaul, David L. Kaplanaccording to the suppliers’ instructions. Plasmids were purified with the GeneJet miniprep (ThermoFisher #K0503) and verified via Sanger sequencing (Genewiz, Cambridge, MA, USA). The transposase plasmid which was

(14) Escherichia coli B-Strains Are Intrinsically Resistant to Colistin and Not Suitable for Characterization and Identification of mcr GenesMicrobiology SpectrumJune 15, 2023Anna Schumann, Alexa R. Cohn, Ahmed Gaballa, Martin Wiedmannpurified using the GeneJet Plasmid Miniprep kit (ThermoFisher Scientific; Waltham, MA; cat. number K0503) and transferred into E. coli SHuffle T7 express (NEB, cat. number C3029), T7 express lysY/lq (NEB,

(15) Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy at active genes in Saccharomyces cerevisiaeNucleic Acids ResearchJune 9, 2023Mitchell A Ellison, Sanchirmaa Namjilsuren, Margaret K Shirra, Matthew S Blacksmith, Rachel A Schusteff, Eleanor M Kerr, Fei Fang, Yufei Xiang, Yi Shi, Karen M Arndtpurified from Escherichia coli DH5α cells using a GeneJET Plasmid Miniprep kit (ThermoFisher Scientific K0503). Plasmids were generated by standard restriction digests, site-directed mutagenesis, or Gibson assembly

(16) Elucidation of the binding mode of organic polysulfides on the human TRPA1 receptorFrontiers in PhysiologyJune 7, 2023Balázs Nemes, Szabolcs László, Balázs Zoltán Zsidó, Csaba Hetényi, Adam Feher, Ferenc Papp, Zoltan Varga, Éva Szőke, Zoltán Sándor, Erika Pintérovernight. The plasmids were isolated by the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific K0503). The plasmid concentration was measured by the Jenway 7310 UV/Vis spectrophotometer. First, the plasmids

(17) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozzion LB plates containing ampicillin. Individual colonies were miniprepped (Thermo Fisher Scientific K0503) and sequenced by Sanger sequencing (Elim Biopharm). Mammalian cell culture HEK293T17 cells were cultured

(18) Loop-mediated isothermal amplification (LAMP)/Cas12a assay for detection of Ralstonia solanacearum in tomatoFrontiers in Bioengineering and BiotechnologyMay 22, 2023Zhiyu Fan, Yuxia Mei, Jiawei Xing, Tian Chen, Di Hu, Hui Liu, Yingjun Li, Derui Liu, Zufeng Liu, Yunxiang LiangPlant Genomic DNA kit (#DP305) were from Tiangen Biotech (Beijing). GeneJET Plasmid Miniprep Kit (#K0503) and Finntip Filtered Pipet Tips (#94052000) were from Thermo Scientific (Waltham, MA, United States).

(19) Optimized metrics for orthogonal combinatorial CRISPR screensScientific ReportsMay 6, 2023Ronay Cetin, Martin Wegner, Leah Luwisch, Sarada Saud, Tatjana Achmedov, Sebastian Süsser, Antonella Vera-Guapi, Konstantin Müller, Yves Matthess, Eva Quandt, et al.culture to obtain the pre-library using the GeneJet Plasmid Miniprep Kit (Thermo Fisher Scientific, K0503) according to the manufacturer's protocol. 3 µg of pre-library was digested with PacI and I-SceI (New England Biolabs

(20) An actin remodeling role for Arabidopsis processing bodies revealed by their proximity interactomeThe EMBO JournalMay 2, 2023Chen Liu, Andriani Mentzelopoulou, Amna Muhammad, Andriy Volkov, Dolf Weijers, Emilio Gutierrez‐Beltran, Panagiotis N MoschoupENTR™/D‐TOPO™ Cloning Kit,Thermo Fisher Scientific,K240020 GeneJET Plasmid Miniprep Kit,ThermoFisher Scientific,K0503 GeneJET Gel Extraction Kit,ThermoFisher Scientific,K0692 Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

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(1)

1/22/2016PCR Purification Kit Kai YThis kit is used to purify PCR products and remove primers and extra nucleotides in the PCR solution. This is a quick and easy to use kit.
(1)

1/21/2016Miniprep Kit Kai YThis kit is used to purify plasmid from bacteria. The kit has simple instructions and produces high quality plasmid with good 260/280 and 260/230 readings. It is also a little cheaper than other kits.... Read Review

GeneJET Plasmid Miniprep Kit from Thermo Fisher Scientific

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Thermo Fisher Scientific

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GeneJET Plasmid Miniprep Kit from Thermo Fisher Scientific

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